The sequence of pDEFEND2 as determined by sequencing:
williams|4582|plasmid prep|restriction|pUC19|pYP
pDefend2 is a plasmid that that encodes for a mutant of yeast-phe tRNA. It was constructed by restricting pDEFEND2 with BSA I and BstBI, followed by ligation of the appropriate sequence to the unique sticky ends. In this mutant the modifications of yeast-phe tRNA have been removed.
The sequence of T7 RNA polymerase
promotor is:
5' AA TTA TAA TAC GAC TCA CTA TA
3'
pDEFEND2 is cut with BsaI and BstBI in order to "open up" the plasmid for insertion of the desired template. BsaI removes four essential bases (ATAG) from the T7 promoter region and therefore must be added to the plus strand of the template.
The sequence of the remainder of
the polylinker is:
5' CGA AGA GCT GAA GCT TGC GTC ATC
CTT 3'
pDEFEND2 sequence
Restriction Enzymes for pDEFEND2
The sequence
of pDEFEND2 as determined by sequencing:
1 GCGCCCAATA CGCAAACCGC
CTCTCCCCGC GCGTTGGCCG ATTCATTAAT
51 GCAGCTGGCA CGACAGGTTT CCCGACTGGA
AAGCGGGCAG TGAGCGCAAC
101 GCAATTAATG TGAGTTAGCT CACTCATTAG
GCACCCCAGG CTTTACACTT
151 TATGCTTCCG GCTCGTATGT TGTGTGGAAT
TGTGAGCGGA TAACAATTTC
201 ACACAGGAAA CAGCTATGAC CATGAATACG
AATTATAATA CGACTCACTA
251 TAGGGAGACC GTCGACGTCT TCGAAGAGCT
GAAGCTTGCG TCATCCTTGG
301 CACTGGCCGT CGTTTTACAA CGTCGTGACT
GGGAAAACCC TGGCGTTACC
351 CAACTTAATC GCCTTGCAGC ACATCCCCCT
TTCGCCAGCT GGCGTAATAG
401 CGAAGAGGCC CGCACCGATC GCCCTTCCCA
ACAGTTGCGC AGCCTGAATG
451 GCGAATGGCG CCTGATGCGG TATTTTCTCC
TTACGCATCT GTGCGGTATT
501 TCACACCGCA TATGGTGCAC TCTCAGTACA
ATCTGCTCTG ATGCCGCATA
551 GTTAAGCCAG CCCCGACACC CGCCAACACC
CGCTGACGCG CCCTGACGGG
601 CTTGTCTGCT CCCGGCATCC GCTTACAGAC
AAGCTGTGAC CGTCTCCGGG
651 AGCTGCATGT GTCAGAGGTT TTCACCGTCA
TCACCGAAAC GCGCGAGACG
701 AAAGGGCCTC GTGATACGCC TATTTTTATA
GGTTAATGTC ATGATAATAA
751 TGGTTTCTTA GCTTTTCGGG GAAATGTGCG
CGGAACCCCT ATTTGTTTAT
801 TTTTCTAAAT ACATTCAAAT ATGTATCCGC
TCATGAGACA ATAACCCTGA
851 TAAATGCTTC AATAATATTG AAAAAGGAAG
AGTATGAGTA TTCAACATTT
901 CCGTGTCGCC CTTATTCCCT TTTTTGCGGC
ATTTTGCCTT CCTGTTTTTG
951 CTCACCCAGA AACGCTGGTG AAAGTAAAAG
ATGCTGAAGA TCAGTTGGGT
1001 GCACGAGTGG GTTACATCGA ACTGGATCTC
AACAGCGGTA AGATCCTTGA
1051 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT
GATGAGCACT TTTAAAGTTC
1101 TGCTATGTGG CGCGGTATTA TCCCGTATTG
ACGCCGGGCA AGAGCAACTC
1151 GGTCGCCGCA TACACTATTC TCAGAATGAC
TTGGTTGAGT ACTCACCAGT
1201 CACAGAAAAG CATCTTACGG ATGGCATGAC
AGTAAGAGAA TTATGCAGTG
1251 CTGCCATAAC CATGAGTGAT AACACTGCGG
CCAACTTACT TCTGACAACG
1301 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT
TTGCACAACA TGGGGGATCA
1351 TGTAACTCGC CTTGATCGTT GGGAACCGGA
GCTGAATGAA GCCATACCAA
1401 ACGACGAGCG TGACACCACG ATGCCTGTAG
CAATGGCAAC AACGTTGCGC
1451 AAACTATTAA CTGGCGAACT ACTTACTCTA
GCTTCCCGGC AACAATTAAT
1501 AGACTGGATG GAGGCGGATA AAGTTGCAGG
ACCACTTCTG CGCTCGGCCC
1551 TTCCGGCTGG CTGGTTTATT GCTGATAAAT
CTGGAGCCGG TGAGCGTGGA
1601 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA
GATGGTAAGC CCTCCCGTAT
1651 CGTAGTTATC TACACGACGG GGAGTCAGGC
AACTATGGAT GAACGAAATA
1701 GACAGATCGC TGAGATAGGT GCCTCACTGA
TTAAGCATTG GTAACTGTCA
1751 GACCAAGTTT ACTCATATAT ACTTTAGATT
GATTTACCCC GGTTGATAAT
1801 CAGAAAAGCC CCAAAAACAG GAAGATTGTA
TAAGCAAATA TTTAAATTGT
1851 AAACGTTAAT ATTTTGTTAA AATTCGCGTT
AAATTTTTGT TAAATCAGCT
1901 CATTTTTTAA CCAATAGGCC GAAATCGGCA
AAATCCCTTA TAAATCAAAA
1951 GAATAGCCCG AGATAGGGTT GAGTGTTGTT
CCAGTTTGGA ACAAGAGTCC
2001 ACTATTAAAG AACGGGACTC CAACGTCAAA
GGGCGAAAAA CCGTCTATCA
2051 GGGCGATGGC CCACTACGTG AACCATCACC
CAAATCAAGT TTTTTGGGGT
2101 CGAGGTGCCG TAAAGCACTA AATCGGAACC
CTAAAGGGAG CCCCCGATTT
2151 AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
GCGAGAAAGG AAGGGAAGAA
2201 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC
AAGTGTAGCG GTCACGCTGC
2251 GCGTAACCAC CACACCCGCC GCGCTTAATG
CGCCGCTACA GGGCGCGTAA
2301 AAGGATCTAG GTGAAGATCC TTTTTGATAA
TCTCATGACC AAAATCCCTT
2351 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG
ACCCCGTAGA AAAGATCAAA
2401 GGATCTTCTT GAGATCCTTT TTTTCTGCGC
GTAATCTGCT GCTTGCAAAC
2451 AAAAAAACCA CCGCTACCAG CGGTGGTTTG
TTTGCCGGAT CAAGAGCTAC
2501 CAACTCTTTT TCCGAAGGTA ACTGGCTTCA
GCAGAGCGCA GATACCAAAT
2551 ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC
CACCACTTCA AGAACTCTGT
2601 AGCACCGCCT ACATACCTCG CTCTGCTAAT
CCTGTTACCA GTGGCTGCTG
2651 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT
TGGACTCAAG ACGATAGTTA
2701 CCGGATAAGG CGCAGCGGTC GGGCTGAACG
GGGGGTTCGT GCACACAGCC
2751 CAGCTTGGAG CGAACGACCT ACACCGAACT
GAGATACCTA CAGCGTGAGC
2801 TATGAGAAAG CGCCACGCTT CCCGAAGGGA
GAAAGCGGAC AGGTATCCGG
2851 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA
CGAGGGAGCT TCCAGGGGGA
2901 AACGCCTGGT ATCTTTATAG TCCTGTCGGG
TTTCGCCACC TCTGACTTGA
2951 GCGTCGATTT TTGTGATGCT CGTCAGGGGG
GCGGAGCCTA TGGAAAAACG
3001 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
CCTTTTGCTG GCCTTTTGCT
3051 CACATGTTCT TTCCTGCGTT ATCCCCTGAT
TCTGTGGATA ACCGTATTAC
3101 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG
CAGCCGAACG ACCGAGCGCA
3151 GCGAGTCAGT GAGCGAGGAA GCGGAAGA
Restriction Enzymes for pDEFEND2 as determined
by Webcutter version
2.0
3178 base pairs
Graphic map | Table by enzyme name | Table by site position
PshBI MspA1I
EaeI AsnI PvuII
gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccga
base pairs
cgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggct
1 to 75
CfrI VspI NspBII
AseI
PshBI
BshNI
AsnI
Eco64I
ctggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactt
base pairs
gacctttcgcccgtcactcgcgttgcgttaattacactcaatcgagtgagtaatccgtggggtccgaaatgtgaa
76 to 150
VspI
BanI
AseI
AccB1I
BstD102I
AccBSI
tatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatga
base pairs
atacgaaggccgagcatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcgatactggtact
151 to 225
BsrBI
HincII BsaHI Bsp119I Ksp632I BssT1I
SalI Msp17I LspI BpuAI Eam1104I StyI
SfcI Eco31I BbiII SfuI Bpu14I
EarI Eco13
atacgaattataatacgactcactatagggagaccgtcgacgtcttcgaagagctgaagcttgcgtcatccttgg
base pairs
tatgcttaatattatgctgagtgatatccctctggcagctgcagaagcttctcgacttcgaacgcagtaggaacc
226 to 300
BstSFI BsaI Hin1I AatII NspV BpiI HindIII
ErhI
AccI Hsp92I Csp45I Bbv16II Eco57I
HindII AcyI BstBI BbsI SapI EcoT14I
0I EaeI
cactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatc
base pairs
gtgaccggcagcaaaatgttgcagcactgacccttttgggaccgcaatgggttgaattagcggaacgtcgtgtag
301 to 375
CfrI
PvuI BsaOI
MspA1I
EarI Ple19I
Acc16I
PvuII
Eam1104I BspCI
FspI BglI
cccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatg
base pairs
ggggaaagcggtcgaccgcattatcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggacttac
376 to 450
NspBII
Ksp632I BsiEI
AviII
Bsh1285I
BstMCI
AccB1I NarI HaeII
Bbv12I
KasI BbiII EheI
VneI BsiHKAI
Eco64I BsaHI BbeI
FauNDI AspHI
gcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctca
base pairs
cgcttaccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtataccacgtgagagt
451 to 525
BanI Msp17I Bsp143II
NdeI Alw44I
BshNI Hsp92I
ApaLI
Hin1I AcyI BstH2I
Alw21I
NspBII
gtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacggg
base pairs
catgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgccc
526 to 600
MspA1I
DrdI
BsmBI NspI
cttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcac
base pairs
gaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtg
601 to 675
Esp3I
EcoO109I
BsmBI BsiI
BspHI
cgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataa
base pairs
gcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattatt
676 to 750
Esp3I BssSI
RcaI
DraII
tggtttcttagcttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgta
base pairs
accaaagaatcgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacat
751 to 825
BstD102I
AccBSI
EarI
BspHI
SspI
Eam1104I
tccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacattt
base pairs
aggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaa
826 to 900
RcaI
Ksp632I
BsrBI
MslI
ccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagt
base pairs
ggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttca
901 to 975
Bbv12I
MflI
ApaLI BssSI
MflI
BstYI
Eco57I Alw44I BsiI
BstYI NspBII
aaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttga
base pairs
ttttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaact
976 to 1050
VneI BsiHKAI
BstX2I MspA1I
AspHI
XhoII
BstX2I
Alw21I
XhoII
DraI
XmnI
Alw21I
Psp1406I AspHI
gagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccg
base pairs
ctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggc
1051 to 1125
Asp700I Bbv12I
BsiHKAI
BsaHI
BcgI
BbiII
BstMCI
Eco255I
Hin1I
BsiEI
Acc113I
tattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagt
base pairs
ataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtca
1126 to 1200
Msp17I
Bsh1285I
ScaI
Hsp92I
BsaOI
AcyI
MslI
cacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacac
base pairs
gtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtg
1201 to 1275
PvuI BsaOI
Ple19I
EaeI
BspCI
tgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatca
base pairs
acgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagt
1276 to 1350
CfrI
BsiEI
Bsh1285I
BstMCI
BsaWI
MslI
tgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcc
base pairs
acattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacgg
1351 to 1425
AviII
PshBI
SfcI
BsrDI Psp1406I
AsnI
tgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaat
base pairs
acatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaatta
1426 to 1500
BstSFI
FspI
VspI
Acc16I
AseI
BglI
agactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctga
base pairs
tctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgact
1501 to 1575
Cfr10I
GsuI MflI
BssAI BstYI
BsrDI
taaatctggagccggtgagcgtggatctcgcggtatcattgcagcactggggccagatggtaagccctcccgtat
base pairs
atttagacctcggccactcgcacctagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcata
1576 to 1650
BsrFI BstX2I
Bse118I XhoII
BpmI
Eam1105I
BshNI
AhdI
Eco64I
cgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctc
base pairs
gcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggag
1651 to 1725
EclHKI
BanI
AspEI
AccB1I
actgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaccccggttgataat
base pairs
tgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaatggggccaactatta
1726 to 1800
SmiI
SspI Psp1406I
AcsI
cagaaaagccccaaaaacaggaagattgtataagcaaatatttaaattgtaaacgttaatattttgttaaaattc
base pairs
gtcttttcggggtttttgtccttctaacatattcgtttataaatttaacatttgcaattataaaacaattttaag
1801 to 1875
SwaI
SspI ApoI
DraI
AcsI
gcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaa
base pairs
cgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccgttttagggaatatttagtttt
1876 to 1950
ApoI
BsoBI
Eco88I
Ama87I
DrdI
gaatagcccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgggactccaacg
base pairs
cttatcgggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgccctgaggttgc
1951 to 2025
BcoI
AvaI
BsaAI
tcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttggggt
base pairs
agtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggtttagttcaaaaaacccca
2026 to 2100
DraIII
NgoAIV
BshNI
BanII
NgoMI
Eco64I
Eco24I
MroNI Cfr1
cgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcga
base pairs
gctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgct
2101 to 2175
BanI
FriOI
BssAI NaeI
AccB1I
BsrFI
Bse118I
HaeII
BstD102I BstH2I
0I
AccBSI Bsp143II
acgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgc
base pairs
tgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacg
2176 to 2250
BsrBI HaeII
Bsp143II
BstH2I
MflI MflI
SfcI
BstYI BstYI
gcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtaaaaggatctaggtgaagatccttttt
base pairs
cgcattggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcattttcctagatccacttctaggaaaaa
2251 to 2325
BstSFI BstX2I
BstX2I
XhoII XhoII
BspHI
gataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaa
base pairs
ctattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagttt
2326 to 2400
RcaI
MflI
MflI
BstYI
BstYI
NspBII
ggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtg
base pairs
cctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccac
2401 to 2475
BstX2I
BstX2I
MspA1I
XhoII
XhoII
Eco57I
gtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaat
base pairs
caaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggttta
2476 to 2550
SfcI
actgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctg
base pairs
tgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagac
2551 to 2625
BstSFI
AlwNI
ctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagtta
base pairs
gattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaat
2626 to 2700
BstMCI
Bbv12I
BsiEI
ApaLI
BsaWI
NspBII
Alw44I
ccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacacc
base pairs
ggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtgg
2701 to 2775
MspA1I
VneI BsiHKAI
Bsh1285I
AspHI
BsaOI
Alw21I
BstH2I
SfcI
Bsp143II
BsaWI
gaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaagcggacaggtatccgg
base pairs
cttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttcgcctgtccataggcc
2776 to 2850
BstSFI
HaeII
BsiI
taagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctg
base pairs
attcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggac
2851 to 2925
BssSI
DrdI
tcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacg
base pairs
agcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgc
2926 to 3000
NspI
BspLU11I
ccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatccc
base pairs
ggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggg
3001 to 3075
AflIII
BstD102I BstMCI
AccBSI BsiEI
ctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgca
base pairs
gactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgt
3076 to 3150
BsrBI Bsh1285I
BsaOI
gcgagtcagtgagcgaggaagcggaaga
base pairs
cgctcagtcactcgctccttcgccttct
3151 to 3178
Table by Enzyme Name
Enzyme
No. Positions
Recognition
name
cuts of sites
sequence
AatII
1 268
gacgt/c
More info
Acc113I
1 1190
agt/act
More in
Acc16I
2 438 1448
tgc/gca
More inf
AccB1I
4 130 457 1718 2104
g/gyrcc
More inf
AccBSI
4 189 832 2214 3126
gagcgg
More inf
AccI
1 262
gt/mkac
More info<
AcsI
2 1870 1881
r/aatty
More info<
AcyI
3 265 458 1131
gr/cgyc
More info<
AflIII
1 3052
a/crygt
More inf
AhdI
1 1671
gacnnn/nngtc More info<
Alw21I
4 519 1003 1088 2743
gwgcw/c
More inf
Alw44I
3 515 999 2739
g/tgcac
More inf
AlwNI
1 2644
cagnnn/ctg More info
Ama87I
1 1957
c/ycgrg
More inf
ApaLI
3 515 999 2739
g/tgcac
More info
ApoI
2 1870 1881
r/aatty
More info<
AseI
3 46 105 1496
at/taat
More info<
AsnI
3 46 105 1496
at/taat
More info<
Asp700I
1 1071
gaann/nnttc More in
AspEI
1 1671
gacnnn/nngtc More info
AspHI
4 519 1003 1088 2743
gwgcw/c
More info
AvaI
1 1957
c/ycgrg
More info<
AviII
2 438 1448
tgc/gca
More info
BanI
4 130 457 1718 2104
g/gyrcc
More info<
BanII
1 2142
grgcy/c
More info
BbeI
1 461
ggcgc/c
More info<
BbiII
3 265 458 1131
gr/cgyc
More info
BbsI
1 272
gaagac
More info<
Bbv12I
4 519 1003 1088 2743
gwgcw/c
More inf
Bbv16II
1 272
gaagac
More in
BcgI
1 1155
cgannnnnntgc More info<
BcoI
1 1957
c/ycgrg
More info<
BglI
2 448 1553
gccnnnn/nggc More info<
BpiI
1 272
gaagac
More info<
BpmI
1 1586
ctggag
More info<
Bpu14I
1 271
tt/cgaa
More inf
BpuAI
1 272
gaagac
More info
BsaAI
1 2067
yac/gtr
More info
BsaHI
3 265 458 1131
gr/cgyc
More info
BsaI
1 260
ggtctc
More info<
BsaOI
5 419 1153 1302 2719 3142
cgry/cg
More info
BsaWI
3 1375 2700 2846
w/ccggw
More info
Bse118I
2 1586 2168
r/ccggy
More in
Bsh1285I
5 419 1153 1302 2719 3142
cgry/cg
More i
BshNI
4 130 457 1718 2104
g/gyrcc
More info
BsiEI
5 419 1153 1302 2719 3142
cgry/cg
More info
BsiHKAI
4 519 1003 1088 2743
gwgcw/c
More in
BsiI
3 713 1007 2884
ctcgtg
More info<
BsmBI
2 646 700
cgtctc
More info
BsoBI
1 1957
c/ycgrg
More info
Bsp119I
1 271
tt/cgaa
More in
Bsp143II
4 461 2218 2226 2813
rgcgc/y
More i
BspCI
2 419 1302
cgat/cg
More info
BspHI
3 739 831 2333
t/catga
More info
BspLU11I
1 3052
a/catgt
More i
BsrBI
4 189 832 2214 3126
gagcgg
More info
BsrDI
2 1435 1617
gcaatg
More info
BsrFI
2 1586 2168
r/ccggy
More info
BssAI
2 1586 2168
r/ccggy
More info
BssSI
3 713 1007 2884
ctcgtg
More info
BssT1I
1 295
c/cwwgg
More inf
BstBI
1 271
tt/cgaa
More info
BstD102I
4 189 832 2214 3126
gagcgg
More i
BstH2I
4 461 2218 2226 2813
rgcgc/y
More inf
BstMCI
5 419 1153 1302 2719 3142
cgry/cg
More inf
BstSFI
5 248 1425 2286 2597 2788
c/tryag
More inf
BstX2I
7 1024 1041 1598 2303 2315 2401 r/gatcy
More inf
2412
BstYI
7 1024 1041 1598 2303 2315 2401 r/gatcy
More info
2412
Cfr10I
2 1586 2168
r/ccggy
More inf
CfrI
3 35 304 1278
y/ggccr
More info<
Csp45I
1 271
tt/cgaa
More inf
DraI
2 1093 1843
ttt/aaa
More info<
DraII
1 704
rg/gnccy More
info
DraIII
1 2067
cacnnn/gtg More inf
DrdI
3 601 2022 2950
gacnnnn/nngtc More info<
EaeI
3 35 304 1278
y/ggccr
More info<
Eam1104I
3 278 407 882
ctcttc
More i
Eam1105I
1 1671
gacnnn/nngtc More i
EarI
3 278 407 882
ctcttc
More info<
EclHKI
1 1671
gacnnn/nngtc More inf
Eco130I
1 295
c/cwwgg
More in
Eco24I
1 2142
grgcy/c
More inf
Eco255I
1 1190
agt/act
More in
Eco31I
1 260
ggtctc
More inf
Eco57I
3 284 989 2531
ctgaag
More inf
Eco64I
4 130 457 1718 2104
g/gyrcc
More inf
Eco88I
1 1957
c/ycgrg
More inf
EcoO109I
1 704
rg/gnccy More
i
EcoT14I
1 295
c/cwwgg
More in
EheI
1 459
ggc/gcc
More info<
ErhI
1 295
c/cwwgg
More info<
Esp3I
2 646 700
cgtctc
More info
FauNDI
1 510
ca/tatg
More inf
FriOI
1 2142
grgcy/c
More info
FspI
2 438 1448
tgc/gca
More info<
GsuI
1 1586
ctggag
More info<
HaeII
4 461 2218 2226 2813
rgcgc/y
More info
Hin1I
3 265 458 1131
gr/cgyc
More info
HincII
1 263
gty/rac
More inf
HindII
1 263
gty/rac
More inf
HindIII
1 282
a/agctt
More in
Hsp92I
3 265 458 1131
gr/cgyc
More inf
KasI
1 457
g/gcgcc
More info<
Ksp632I
3 278 407 882
ctcttc
More in
LspI
1 271
tt/cgaa
More info<
MflI
7 1024 1041 1598 2303 2315 2401 r/gatcy
More info<
2412
MroNI
1 2168
g/ccggc
More info
MslI
3 900 1259 1418
caynn/nnrtg More info<
Msp17I
3 265 458 1131
gr/cgyc
More inf
MspA1I
6 54 388 582 1035 2470 2715
cmg/ckg
More inf
NaeI
1 2170
gcc/ggc
More info<
NarI
1 458
gg/cgcc
More info<
NdeI
1 510
ca/tatg
More info<
NgoAIV
1 2168
g/ccggc
More inf
NgoMI
1 2168
g/ccggc
More info
NspBII
6 54 388 582 1035 2470 2715
cmg/ckg
More inf
NspI
2 659 3056
rcatg/y
More info<
NspV
1 271
tt/cgaa
More info<
Ple19I
2 419 1302
cgat/cg
More inf
PshBI
3 46 105 1496
at/taat
More info
Psp1406I
3 1069 1442 1853
aa/cgtt
More i
PvuI
2 419 1302
cgat/cg
More info<
PvuII
2 54 388
cag/ctg
More info
RcaI
3 739 831 2333
t/catga
More info<
SalI
1 261
g/tcgac
More info<
SapI
1 279
gctcttc
More info<
ScaI
1 1190
agt/act
More info<
SfcI
5 248 1425 2286 2597 2788
c/tryag
More info<
SfuI
1 271
tt/cgaa
More info<
SmiI
1 1843
attt/aaat More info<
SspI
3 866 1839 1860
aat/att
More info<
StyI
1 295
c/cwwgg
More info<
SwaI
1 1843
attt/aaat More info<
VneI
3 515 999 2739
g/tgcac
More info<
VspI
3 46 105 1496
at/taat
More info<
XhoII
7 1024 1041 1598 2303 2315 2401 r/gatcy
More info
2412
XmnI
1 1071
gaann/nnttc More info<
The following endonucleases
were selected but don't cut this sequence:
AatI, Acc65I, AccB7I,
AccIII, AclNI, AfeI, AflII, AgeI, AocI, Aor51HI,
ApaI, AscI, Asp718I,
AspI, AtsI, AvrII, BalI, BamHI, BanIII, BbrPI, BbuI,
BclI, BfrI, BglII, BlnI,
BlpI, Bpu1102I, Bsa29I, BsaBI, BsaMI, BscI, Bse21I,
Bse8I, BseAI, BseCI,
BsePI, BseRI, BsgI, Bsh1365I, BsiMI, BsiWI, BsmI,
Bsp106I, Bsp120I, Bsp13I,
Bsp1407I, Bsp1720I, Bsp19I, Bsp68I, BspDI, BspEI,
BspMI, BspTI, BspXI,
BsrBRI, BsrGI, BssHII, Bst1107I, Bst98I, BstDSI, BstEII,
BstI, BstPI, BstSNI,
BstXI, BstZI, Bsu15I, Bsu36I, CciNI, CelII, Cfr42I,
Cfr9I, ClaI, CpoI, CspI,
CvnI, DsaI, EagI, Ecl136II, EclXI, Eco105I, Eco147I,
Eco32I, Eco47III, Eco52I,
Eco72I, Eco81I, Eco91I, EcoICRI, EcoNI, EcoO65I,
EcoRI, EcoRV, EcoT22I,
Esp1396I, FbaI, FseI, HpaI, Kpn2I, KpnI, Ksp22I,
KspI, MamI, MfeI, MluI,
MluNI, Mph1103I, MroI, MscI, MspCI, MunI, Mva1269I,
NcoI, NheI, NotI, NruI,
NsiI, PacI, PaeI, PaeR7I, Pfl23II, PflMI, PinAI,
PmaCI, Pme55I, PmeI,
PmlI, Ppu10I, PpuMI, PshAI, Psp124BI, Psp5II, PspAI,
PspALI, PspEI, PspLI,
PspOMI, PstI, PstNHI, RsrII, SacI, SacII, SbfI, SexAI,
SfiI, Sfr274I, Sfr303I,
SgfI, SgrAI, SmaI, SnaBI, SpeI, SphI, SplI, SrfI,
Sse8387I, SseBI, SspBI,
SstI, SstII, StuI, SunI, Tth111I, Van91I, Vha464I,
XbaI, XcmI, XhoI, XmaI,
XmaIII, Zsp2I
Table by Site Position
Cut
Enzyme No. Positions
Recognition
site
name cuts of other
sites sequence
35
EaeI 3
304 1278
y/ggccr More
info
35
CfrI 3
304 1278
y/ggccr More
info
46
AsnI 3
105 1496
at/taat More
info
46
VspI 3
105 1496
at/taat More
info
46
PshBI 3
105 1496
at/taat More
info
46
AseI 3
105 1496
at/taat More
info
54
PvuII 2
388
cag/ctg More
info
54
NspBII 6 388
582 1035 2470 2715 cmg/ckg
More info
54
MspA1I 6 388
582 1035 2470 2715 cmg/ckg
More info
105
AsnI 3
46 1496
at/taat More
info
105
VspI 3
46 1496
at/taat More
info
105
PshBI 3
46 1496
at/taat More
info
105
AseI 3
46 1496
at/taat More
info
130
Eco64I 4 457
1718 2104 g/gyrcc
More info
130
BanI 4
457 1718 2104 g/gyrcc
More info
130
BshNI 4
457 1718 2104 g/gyrcc
More info
130
AccB1I 4 457
1718 2104 g/gyrcc
More info
189
AccBSI 4 832
2214 3126 gagcgg
More info
189
BsrBI 4
832 2214 3126 gagcgg
More info
189
BstD102I 4 832 2214 3126
gagcgg
More info
248
SfcI 5
1425 2286 2597 2788 c/tryag
More info
248
BstSFI 5 1425
2286 2597 2788 c/tryag
More info
260
Eco31I 1
ggtctc
More info
260
BsaI 1
ggtctc
More info
261
SalI 1
g/tcgac More
info
262
AccI 1
gt/mkac More
info
263
HincII 1
gty/rac More
info
263
HindII 1
gty/rac More
info
265
Hin1I 3
458 1131
gr/cgyc More
info
265
Msp17I 3 458
1131
gr/cgyc More
info
265
BbiII 3
458 1131
gr/cgyc More
info
265
Hsp92I 3 458
1131
gr/cgyc More
info
265
BsaHI 3
458 1131
gr/cgyc More
info
265
AcyI 3
458 1131
gr/cgyc More
info
268
AatII 1
gacgt/c More
info
271
SfuI 1
tt/cgaa More
info
271
LspI 1
tt/cgaa More
info
271
Csp45I 1
tt/cgaa More
info
271
BstBI 1
tt/cgaa More
info
271
Bsp119I 1
tt/cgaa More
info
271
NspV 1
tt/cgaa More
info
271
Bpu14I 1
tt/cgaa More
info
272
BpuAI 1
gaagac
More info
272
Bbv16II 1
gaagac
More info
272
BbsI 1
gaagac
More info
272
BpiI 1
gaagac
More info
278
Eam1104I 3 407 882
ctcttc
More info
278
Ksp632I 3 407 882
ctcttc
More info
278
EarI 3
407 882
ctcttc
More info
279
SapI 1
gctcttc More
info
282
HindIII 1
a/agctt More
info
284
Eco57I 3 989
2531
ctgaag
More info
295
Eco130I 1
c/cwwgg More
info
295
ErhI 1
c/cwwgg More
info
295
StyI 1
c/cwwgg More
info
295
BssT1I 1
c/cwwgg More
info
295
EcoT14I 1
c/cwwgg More
info
304
EaeI 3
35 1278
y/ggccr More
info
304
CfrI 3
35 1278
y/ggccr More
info
388
PvuII 2
54
cag/ctg More
info
388
NspBII 6 54
582 1035 2470 2715 cmg/ckg
More info
388
MspA1I 6 54
582 1035 2470 2715 cmg/ckg
More info
407
Eam1104I 3 278 882
ctcttc
More info
407
Ksp632I 3 278 882
ctcttc
More info
407
EarI 3
278 882
ctcttc
More info
419
BspCI 2
1302
cgat/cg More
info
419
BsiEI 5
1153 1302 2719 3142 cgry/cg
More info
419
Ple19I 2 1302
cgat/cg More
info
419
Bsh1285I 5 1153 1302 2719
3142 cgry/cg
More info
419
PvuI 2
1302
cgat/cg More
info
419
BstMCI 5 1153
1302 2719 3142 cgry/cg
More info
419
BsaOI 5
1153 1302 2719 3142 cgry/cg
More info
438
FspI 2
1448
tgc/gca More
info
438
AviII 2
1448
tgc/gca More
info
438
Acc16I 2 1448
tgc/gca More
info
448
BglI 2
1553
gccnnnn/nggc More info
457
Eco64I 4 130
1718 2104 g/gyrcc
More info
457
BanI 4
130 1718 2104 g/gyrcc
More info
457
KasI 1
g/gcgcc More
info
457
BshNI 4
130 1718 2104 g/gyrcc
More info
457
AccB1I 4 130
1718 2104 g/gyrcc
More info
458
Hin1I 3
265 1131
gr/cgyc More
info
458
Msp17I 3 265
1131
gr/cgyc More
info
458
BbiII 3
265 1131
gr/cgyc More
info
458
Hsp92I 3 265
1131
gr/cgyc More
info
458
BsaHI 3
265 1131
gr/cgyc More
info
458
NarI 1
gg/cgcc More
info
458
AcyI 3
265 1131
gr/cgyc More
info
459
EheI 1
ggc/gcc More
info
461
Bsp143II 4 2218 2226 2813
rgcgc/y More
info
461
HaeII 4
2218 2226 2813 rgcgc/y
More info
461
BstH2I 4 2218
2226 2813 rgcgc/y
More info
461
BbeI 1
ggcgc/c More
info
510
FauNDI 1
ca/tatg More
info
510
NdeI 1
ca/tatg More
info
515
Alw44I 3 999
2739
g/tgcac More
info
515
VneI 3
999 2739
g/tgcac More
info
515
ApaLI 3
999 2739
g/tgcac More
info
519
AspHI 4
1003 1088 2743 gwgcw/c
More info
519
Bbv12I 4 1003
1088 2743 gwgcw/c
More info
519
Alw21I 4 1003
1088 2743 gwgcw/c
More info
519
BsiHKAI 4 1003 1088
2743 gwgcw/c
More info
582
NspBII 6 54
388 1035 2470 2715 cmg/ckg
More info
582
MspA1I 6 54
388 1035 2470 2715 cmg/ckg
More info
601
DrdI 3
2022 2950
gacnnnn/nngtc More info
646
BsmBI 2
700
cgtctc
More info
646
Esp3I 2
700
cgtctc
More info
659
NspI 2
3056
rcatg/y More
info
700
BsmBI 2
646
cgtctc
More info
700
Esp3I 2
646
cgtctc
More info
704
EcoO109I 1
rg/gnccy More info
704
DraII 1
rg/gnccy More info
713
BsiI 3
1007 2884
ctcgtg
More info
713
BssSI 3
1007 2884
ctcgtg
More info
739
BspHI 3
831 2333
t/catga More
info
739
RcaI 3
831 2333
t/catga More
info
831
BspHI 3
739 2333
t/catga More
info
831
RcaI 3
739 2333
t/catga More
info
832
AccBSI 4 189
2214 3126 gagcgg
More info
832
BsrBI 4
189 2214 3126 gagcgg
More info
832
BstD102I 4 189 2214 3126
gagcgg
More info
866
SspI 3
1839 1860
aat/att More
info
882
Eam1104I 3 278 407
ctcttc
More info
882
Ksp632I 3 278 407
ctcttc
More info
882
EarI 3
278 407
ctcttc
More info
900
MslI 3
1259 1418
caynn/nnrtg More info
989
Eco57I 3 284
2531
ctgaag
More info
999
Alw44I 3 515
2739
g/tgcac More
info
999
VneI 3
515 2739
g/tgcac More
info
999
ApaLI 3
515 2739
g/tgcac More
info
1003
AspHI 4
519 1088 2743 gwgcw/c
More info
1003
Bbv12I 4 519
1088 2743 gwgcw/c
More info
1003
Alw21I 4 519
1088 2743 gwgcw/c
More info
1003
BsiHKAI 4 519 1088
2743 gwgcw/c
More info
1007
BsiI 3
713 2884
ctcgtg
More info
1007
BssSI 3
713 2884
ctcgtg
More info
1024
BstYI 7
1041 1598 2303 2315 r/gatcy
More info
2401 2412
1024
BstX2I 7 1041
1598 2303 2315 r/gatcy
More info
2401 2412
1024
MflI 7
1041 1598 2303 2315 r/gatcy
More info
2401 2412
1024
XhoII 7
1041 1598 2303 2315 r/gatcy
More info
2401 2412
1035
NspBII 6 54
388 582 2470 2715 cmg/ckg
More info
1035
MspA1I 6 54
388 582 2470 2715 cmg/ckg
More info
1041
BstYI 7
1024 1598 2303 2315 r/gatcy
More info
2401 2412
1041
BstX2I 7 1024
1598 2303 2315 r/gatcy
More info
2401 2412
1041
MflI 7
1024 1598 2303 2315 r/gatcy
More info
2401 2412
1041
XhoII 7
1024 1598 2303 2315 r/gatcy
More info
2401 2412
1069
Psp1406I 3 1442 1853
aa/cgtt More
info
1071
Asp700I 1
gaann/nnttc More info
1071
XmnI 1
gaann/nnttc More info
1088
AspHI 4
519 1003 2743 gwgcw/c
More info
1088
Bbv12I 4 519
1003 2743 gwgcw/c
More info
1088
Alw21I 4 519
1003 2743 gwgcw/c
More info
1088
BsiHKAI 4 519 1003
2743 gwgcw/c
More info
1093
DraI 2
1843
ttt/aaa More
info
1131
Hin1I 3
265 458
gr/cgyc More
info
1131
Msp17I 3 265
458
gr/cgyc More
info
1131
BbiII 3
265 458
gr/cgyc More
info
1131
Hsp92I 3 265
458
gr/cgyc More
info
1131
BsaHI 3
265 458
gr/cgyc More
info
1131
AcyI 3
265 458
gr/cgyc More
info
1153
BsiEI 5
419 1302 2719 3142 cgry/cg
More info
1153
Bsh1285I 5 419 1302 2719
3142 cgry/cg
More info
1153
BstMCI 5 419
1302 2719 3142 cgry/cg
More info
1153
BsaOI 5
419 1302 2719 3142 cgry/cg
More info
1155
BcgI 1
cgannnnnntgc More info
1190
Acc113I 1
agt/act More
info
1190
ScaI 1
agt/act More
info
1190
Eco255I 1
agt/act More
info
1259
MslI 3
900 1418
caynn/nnrtg More info
1278
EaeI 3
35 304
y/ggccr More
info
1278
CfrI 3
35 304
y/ggccr More
info
1302
BspCI 2
419
cgat/cg More
info
1302
BsiEI 5
419 1153 2719 3142 cgry/cg
More info
1302
Ple19I 2 419
cgat/cg More
info
1302
Bsh1285I 5 419 1153 2719
3142 cgry/cg
More info
1302
PvuI 2
419
cgat/cg More
info
1302
BstMCI 5 419
1153 2719 3142 cgry/cg
More info
1302
BsaOI 5
419 1153 2719 3142 cgry/cg
More info
1375
BsaWI 3
2700 2846
w/ccggw More
info
1418
MslI 3
900 1259
caynn/nnrtg More info
1425
SfcI 5
248 2286 2597 2788 c/tryag
More info
1425
BstSFI 5 248
2286 2597 2788 c/tryag
More info
1435
BsrDI 2
1617
gcaatg
More info
1442
Psp1406I 3 1069 1853
aa/cgtt More
info
1448
FspI 2
438
tgc/gca More
info
1448
AviII 2
438
tgc/gca More
info
1448
Acc16I 2 438
tgc/gca More
info
1496
AsnI 3
46 105
at/taat More
info
1496
VspI 3
46 105
at/taat More
info
1496
PshBI 3
46 105
at/taat More
info
1496
AseI 3
46 105
at/taat More
info
1553
BglI 2
448
gccnnnn/nggc More info
1586
BssAI 2
2168
r/ccggy More
info
1586
BsrFI 2
2168
r/ccggy More
info
1586
GsuI 1
ctggag
More info
1586
Bse118I 2 2168
r/ccggy More
info
1586
Cfr10I 2 2168
r/ccggy More
info
1586
BpmI 1
ctggag
More info
1598
BstYI 7
1024 1041 2303 2315 r/gatcy
More info
2401 2412
1598
BstX2I 7 1024
1041 2303 2315 r/gatcy
More info
2401 2412
1598
MflI 7
1024 1041 2303 2315 r/gatcy
More info
2401 2412
1598
XhoII 7
1024 1041 2303 2315 r/gatcy
More info
2401 2412
1617
BsrDI 2
1435
gcaatg
More info
1671
AhdI 1
gacnnn/nngtc More info
1671
EclHKI 1
gacnnn/nngtc More info
1671
Eam1105I 1
gacnnn/nngtc More info
1671
AspEI 1
gacnnn/nngtc More info
1718
Eco64I 4 130
457 2104 g/gyrcc
More info
1718
BanI 4
130 457 2104
g/gyrcc More
info
1718
BshNI 4
130 457 2104
g/gyrcc More
info
1718
AccB1I 4 130
457 2104 g/gyrcc
More info
1839
SspI 3
866 1860
aat/att More
info
1843
SwaI 1
attt/aaat More info
1843
SmiI 1
attt/aaat More info
1843
DraI 2
1093
ttt/aaa More
info
1853
Psp1406I 3 1069 1442
aa/cgtt More
info
1860
SspI 3
866 1839
aat/att More
info
1870
AcsI 2
1881
r/aatty More
info
1870
ApoI 2
1881
r/aatty More
info
1881
AcsI 2
1870
r/aatty More
info
1881
ApoI 2
1870
r/aatty More
info
1957
Ama87I 1
c/ycgrg More
info
1957
BcoI 1
c/ycgrg More
info
1957
Eco88I 1
c/ycgrg More
info
1957
AvaI 1
c/ycgrg More
info
1957
BsoBI 1
c/ycgrg More
info
2022
DrdI 3
601 2950
gacnnnn/nngtc More info
2067
BsaAI 1
yac/gtr More
info
2067
DraIII 1
cacnnn/gtg More info
2104
Eco64I 4 130
457 1718 g/gyrcc
More info
2104
BanI 4
130 457 1718
g/gyrcc More
info
2104
BshNI 4
130 457 1718
g/gyrcc More
info
2104
AccB1I 4 130
457 1718 g/gyrcc
More info
2142
Eco24I 1
grgcy/c More
info
2142
FriOI 1
grgcy/c More
info
2142
BanII 1
grgcy/c More
info
2168
MroNI 1
g/ccggc More
info
2168
BssAI 2
1586
r/ccggy More
info
2168
NgoMI 1
g/ccggc More
info
2168
BsrFI 2
1586
r/ccggy More
info
2168
NgoAIV 1
g/ccggc More
info
2168
Bse118I 2 1586
r/ccggy More
info
2168
Cfr10I 2 1586
r/ccggy More
info
2170
NaeI 1
gcc/ggc More
info
2214
AccBSI 4 189
832 3126 gagcgg
More info
2214
BsrBI 4
189 832 3126
gagcgg
More info
2214
BstD102I 4 189 832 3126
gagcgg
More info
2218
Bsp143II 4 461 2226 2813
rgcgc/y More
info
2218
HaeII 4
461 2226 2813 rgcgc/y
More info
2218
BstH2I 4 461
2226 2813 rgcgc/y
More info
2226
Bsp143II 4 461 2218 2813
rgcgc/y More
info
2226
HaeII 4
461 2218 2813 rgcgc/y
More info
2226
BstH2I 4 461
2218 2813 rgcgc/y
More info
2286
SfcI 5
248 1425 2597 2788 c/tryag
More info
2286
BstSFI 5 248
1425 2597 2788 c/tryag
More info
2303
BstYI 7
1024 1041 1598 2315 r/gatcy
More info
2401 2412
2303
BstX2I 7 1024
1041 1598 2315 r/gatcy
More info
2401 2412
2303
MflI 7
1024 1041 1598 2315 r/gatcy
More info
2401 2412
2303
XhoII 7
1024 1041 1598 2315 r/gatcy
More info
2401 2412
2315
BstYI 7
1024 1041 1598 2303 r/gatcy
More info
2401 2412
2315
BstX2I 7 1024
1041 1598 2303 r/gatcy
More info
2401 2412
2315
MflI 7
1024 1041 1598 2303 r/gatcy
More info
2401 2412
2315
XhoII 7
1024 1041 1598 2303 r/gatcy
More info
2401 2412
2333
BspHI 3
739 831
t/catga More
info
2333
RcaI 3
739 831
t/catga More
info
2401
BstYI 7
1024 1041 1598 2303 r/gatcy
More info
2315 2412
2401
BstX2I 7 1024
1041 1598 2303 r/gatcy
More info
2315 2412
2401
MflI 7
1024 1041 1598 2303 r/gatcy
More info
2315 2412
2401
XhoII 7
1024 1041 1598 2303 r/gatcy
More info
2315 2412
2412
BstYI 7
1024 1041 1598 2303 r/gatcy
More info
2315 2401
2412
BstX2I 7 1024
1041 1598 2303 r/gatcy
More info
2315 2401
2412
MflI 7
1024 1041 1598 2303 r/gatcy
More info
2315 2401
2412
XhoII 7
1024 1041 1598 2303 r/gatcy
More info
2315 2401
2470
NspBII 6 54
388 582 1035 2715 cmg/ckg
More info
2470
MspA1I 6 54
388 582 1035 2715 cmg/ckg
More info
2531
Eco57I 3 284
989
ctgaag
More info
2597
SfcI 5
248 1425 2286 2788 c/tryag
More info
2597
BstSFI 5 248
1425 2286 2788 c/tryag
More info
2644
AlwNI 1
cagnnn/ctg More info
2700
BsaWI 3
1375 2846
w/ccggw More
info
2715
NspBII 6 54
388 582 1035 2470 cmg/ckg
More info
2715
MspA1I 6 54
388 582 1035 2470 cmg/ckg
More info
2719
BsiEI 5
419 1153 1302 3142 cgry/cg
More info
2719
Bsh1285I 5 419 1153 1302
3142 cgry/cg
More info
2719
BstMCI 5 419
1153 1302 3142 cgry/cg
More info
2719
BsaOI 5
419 1153 1302 3142 cgry/cg
More info
2739
Alw44I 3 515
999
g/tgcac More
info
2739
VneI 3
515 999
g/tgcac More
info
2739
ApaLI 3
515 999
g/tgcac More
info
2743
AspHI 4
519 1003 1088 gwgcw/c
More info
2743
Bbv12I 4 519
1003 1088 gwgcw/c
More info
2743
Alw21I 4 519
1003 1088 gwgcw/c
More info
2743
BsiHKAI 4 519 1003
1088 gwgcw/c
More info
2788
SfcI 5
248 1425 2286 2597 c/tryag
More info
2788
BstSFI 5 248
1425 2286 2597 c/tryag
More info
2813
Bsp143II 4 461 2218 2226
rgcgc/y More
info
2813
HaeII 4
461 2218 2226 rgcgc/y
More info
2813
BstH2I 4 461
2218 2226 rgcgc/y
More info
2846
BsaWI 3
1375 2700
w/ccggw More
info
2884
BsiI 3
713 1007
ctcgtg
More info
2884
BssSI 3
713 1007
ctcgtg
More info
2950
DrdI 3
601 2022
gacnnnn/nngtc More info
3052
BspLU11I 1
a/catgt More
info
3052
AflIII 1
a/crygt More
info
3056
NspI 2
659
rcatg/y More
info
3126
AccBSI 4 189
832 2214 gagcgg
More info
3126
BsrBI 4
189 832 2214
gagcgg
More info
3126
BstD102I 4 189 832 2214
gagcgg
More info
3142
BsiEI 5
419 1153 1302 2719 cgry/cg
More info
3142
Bsh1285I 5 419 1153 1302
2719 cgry/cg
More info
3142
BstMCI 5 419
1153 1302 2719 cgry/cg
More info
3142
BsaOI 5
419 1153 1302 2719 cgry/cg
More info